For this task we recommend using one of two software packages freely available online.įirst and easiest to use is the web-based version of the Decipher chimera detection tool at. The sequence files produced by the initial processing tool need to be checked for chimeras. Then follow the Pipeline Initial Processing tutorial to begin processing your data. Prepare your input including a sequence file in FASTA, FASTQ or SFF format, a primer sequence and a tag file. ![]() You may follow this tutorial using your own sequences or you may download and use the input files provided in the Pipeline Initial Processing tutorial. Supervised processing and analysis of 16S rRNA data starts with the initial processing tool. ![]() Initial processing -> Chimera removal -> Taxonomic Classification -> Analysis Step 1: Initial Processing
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